肺臟，肝臟，脾臟三個(gè)部位經(jīng)常是動(dòng)物實(shí)驗(yàn)需要檢測的部位，如何通過流式檢測肺臟，肝臟，脾臟三個(gè)部位的巨噬細(xì)胞？首要的是流式圈門，肺肝脾巨噬細(xì)胞圈門策略文獻(xiàn)參考，可以參考如下文獻(xiàn)。

論文信息：

論文題目：Microbiota-induced EI24 improves homeostasis but impedes function of alveolar macrophages via metabolic regulation

期刊名稱：Nature Communications

時(shí)間期卷：17, Article number: 2227(2026)

在線時(shí)間：2026年1月31日

DOI： doi.org/10.1038/s41467-026-68466-5

Clodronate Liposomes氯膦酸鹽脂質(zhì)體在小鼠病毒感染和腫瘤轉(zhuǎn)移模型清除肺泡巨噬細(xì)胞和外周單核巨噬細(xì)胞。荷蘭Liposoma巨噬細(xì)胞清除劑ClodronateLiposomes見刊于Nature Communications：微生物群誘導(dǎo)的EI24通過代謝調(diào)控改善穩(wěn)態(tài)，但阻礙肺泡巨噬細(xì)胞功能。

肺肝脾巨噬細(xì)胞圈門策略文獻(xiàn)參考：

a/肺臟：Gating strategy used for identification of lung AMs and IMs.

                 AMs were identified as CD45+ CD11b1o CD11c+ F4/80+ SiglecF+

                 IMs were characterized as CD45+CD11b+CD11c- F4/80+.

b/肝臟： Gating strategy of liver macrophages.

                  Liver macrophages wereidentified as CD45+ F4/80+ CD11b+

c/脾臟： Gating strategy of spleen RPMs.

                 RPMs wereidentified as CD45+ F4/80+ CD11bint